Marmoset sequence unveils new information on primate biology and evolution
A group of scientists from around the globe led by the Baylor College of Medicine and Washington University in St. Louis has completed the genome sequence of the common marmoset- the first sequence of a New World Monkey- providing brand new information on the marmoset’s unique rapid reproductive system, physiology and growth, unveiling new information on primate biology and evolution.
The team published the work in the journal Nature Genetics.
“We study primate genomes to get a better understanding of the biology of the species that are most closely related to humans,” said Dr. Jeffrey Rogers, associate professor in the Human Genome Sequencing Center at Baylor and a lead author on the report. “The previous sequences of the great apes and macaques, which are very closely related to humans on the primate evolutionary tree, have provided remarkable new information about the evolutionary origins of the human genome and the processes involved.”
By sequencing the genomes of the marmoset, the team revealed for the first time the genome of a non-human primate in the New World monkeys, which represents a separate branch in the primate evolutionary tree that is more distant from humans than primates whose genomes have been sequenced in detail previously. The sequence has allowed scientists to broaden their ability to study the human genome and its history as revealed by comparison with other primates.
The sequencing was conducted by Baylor and Washington University and led by Dr. Kim Worley, a professor in the Human Genome Sequencing Centre, along with Rogers at Baylor, and Drs. Richard K. Wilson, director, and Wesley Warren of the Genome Institute at Washington University, in collaboration with Dr. Suzette Tardif of the University of Texas Health Science Centre in San Antonio and the Southwest National Primate Research Centre.
“Each new non-human primate genome adds to a deeper understanding of human biology,” said Dr. Richard Gibbs, director of the Human Genome Sequencing Center at Baylor and a principal investigator of the study.
The study unveiled unique genetic characteristics observed in the marmoset, including several genes that are probably responsible for their ability to regularly produce twins.
“Unlike humans, marmosets consistently give birth to twins without the association of any medical issues,” said Worley. “So why is it OK in marmosets but not in humans where it is considered high risk and associated with more complications?”
It has been revealed that the marmoset gene WFIKKN1 exhibits changes associated with twinning in marmosets.
“From our analysis it appears that the gene may act as some kind of critical switch between multiples and singleton pregnancies, though it is not the only gene involved,” said Rogers, who also stated the finding could apply to studies of multiple pregnancies in humans.
The team was also researching genetic changes associated with a unique trait found in marmosets and their close relatives, but not described in any other mammal. The dizygotic, otherwise known as fraternal, twins in marmosets exchange blood stem cells called hematopoietic stem cells in utero, which leads to chimerism, a single organism composed of genetically distinct cells.
“This is very unusual. The twins are full siblings, but if you draw a blood sample from one animal, between 10 and 50 percent of the cells will carry the sibling’s DNA,” said Rogers. “Normally, fraternal twins do not share the same DNA in this way, and in other animals, this chimerism can cause medical problems but not in marmosets. It is very unique.”
“The translational implications of this work to pregnancy and reproductive medicine are significant. We have shown that there are several genes in the marmoset, which likely enable twinning. However, it is not just a question of why they have such a high rate of twinning, but how do they manage to rear and raise these twins so successfully,” said Dr. Kjersti Aagaard, associate professor of obstetrics and gynecology – maternal fetal medicine at Baylor and a co-author on the study. “Given the relatively high rate of complications of twins we see, ranging from preterm birth to unique complications such as Twin Twin Transfusion Syndrome (seen only amongst identical or monochorionic twins), it is crucial to understand the underlying adaptive biology of the marmoset which enables them to avoid these complications.”
Marmosets are members of a unique social system. The dominant male and female serve as the primary breeders for the family, while their relatives aid in caring for the offspring. They pick them up; carry them for long periods, thus basically providing all the support in order to enable the breeders to reproduce again quickly. Fascinatingly, the relatives who provide the care are reproductively suppressed, according to Worley.
“This species is clearly adapted to rapid reproduction and to the potential for rapid population expansion,” said Rogers. “Their ecological system connects with that as they are able to thrive in disturbed areas of forests. So one possibility is that they have evolved a feeding and dietary regimen that allows them to live in these type of conditions where they can reproduce quickly. This would be advantageous as any adults that move into a newly disturbed area would establish their offspring as the early initial residents of the newly available area.”
Small body size
Marmosets are also known for their small body size. The genome sequence showed this might be as a result of positive selection in five growth hormone/insulin-like growth factor axis genes (GH-IGF) with potential roles in producing small body size.
In addition to this, the team identified a cluster of genes that affect metabolic rates and body temperatures, adaptations associated with challenges of small body size.
The study also provides new information about microRNAs, small non-coding RNA molecules that function to regulate gene expression.
“There has not been much research conducted on microRNAs in nonhuman primates, so we found this particularly important,” said Worley.
A team led by Dr. Preethi Gunarantne, an associate professor of biology and biochemistry at the University of Houston and of pathology and a member of the Human Genome Sequencing Centre at Baylor, along with Dr. R. Alan Harris, an assistant professor of molecular and human genetics at Baylor, discovered that marmosets exhibit a large number of differences in microRNAs and their gene targets in comparison with humans, with two large clusters potentially involved in reproduction.
The sequence forms the starting blocks for further biomedical research using marmosets, said Rogers.
“Researchers may have been more reluctant to study the marmoset due to lack of basic information, but this genome sequence opens new avenues for future research relevant to various aspects of human health and disease.”
Dr. Suzette Tardiff, professor of cellular and structural biology at the Barshop Institute for Longevity and Aging Studies at The University of Texas Health Science Centre at San Antonio, a core scientist at the Southwest National Primate Research Centre and an expert in marmoset biology and co-author of the paper, provided vital information regarding the biology of marmosets, and helped gather samples for the sequence.
Contributing Source: Baylor College of Medicine
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